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KMID : 0616120120430030250
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 3 p.250 ~ p.256
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Solid-Phase Extraction of Curcuminoid from Turmeric Using Physical Process Method
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Lee Kwang-Jin
Yang Hye-Jin
Jeong Sang-Won
Ma Jin-Yeul
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Abstract
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In order to extract the curcuminoid such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric (Curcuma longa), solvent extraction methods (dipping and ultrasonic extraction method) and solid-phase extraction (SPE) were used. RP-HPLC (reverse-phase high-performance liquid chromatography) and TLC (thin-layer chromatography) were used for identification and analysis the three curcuminoid. From the experimental results, it is evident that the percentage of curcuminoid extracted from turmeric by ultrasonic extraction method was higher than dipping method. The percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanolic composition. Moreover, the total peak area of three curcuminoid was above 92% in RP-HPLC using solid-phase extraction. These results will form a database for investigating the constituents of natural products and the resources of pharmaceutical, nutrition, and cosmetic products.
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KEYWORD
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Turmeric, Curcuminoid, Solid-Phase Extraction, Ultrasonic wave
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